bacterial transformation lab answers

Course Hero is not sponsored or endorsed by any college or university. From the results that you obtained, how could you prove that these changes that occurred were due to the procedure that you performed? Genetic transformation occurs when a cell takes up and expresses a new piece or foreign ____________, often a circular plasmid. When a bacterial cell freezes, the volume of cytoplasm expands. The source of fluorescence is probably from some protein that the plasmid encodes from the addition of arabinose, namely GFP. Learn. Look again at your four plates. The questions go in the same order that they are asked in the PDF so make sure to answer these questions while you read the lab PDF. Explain your answer. Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies. Bacteria which resemble the non-transformed will be found on the LB/(-) pGLO plate. Biology Bacterial Transformation Virtual Lab Classzone Answers Read Free Bacterial Transformation Virtual Lab Classzone Answers. In this experiment, bacteria will be transformed with a gene that codes for Green Fluorescent Protein (GFP). What was the purpose of rupturing or lysing the bacteria? Recall that the goal of genetic transformation is to change an organism's traits (phenotype). pGlo plasmids, when taken up by a bacteria, will code for. Bacterial Transformation LAB Analyzing Results. Introduction: Bacterial transformation occurs when a bacterial cell takes up foreign DNA and incorporates it into its own DNA. Bacteria are small, single-celled organisms which reproduce quickly and easily. Table of Contents:00:26 - Central Framework/Dogma of Molecular Biology00:58 - Bacterial Transformation02:59 - 04:11 - 06:22 - Since only some of the cells exposed to the amp R plasmids will actually take them in, only some cells will be transformed. Match. If there are any genetically transformed bacterial cells, on which plate(s) would they most likely be located? The UV light is necessary to cause the GFP protein within the bacteria to fluoresce. Based on the above considerations, which would be the best choice for a genetic transformation: a bacterium, earthworm, fish, or mouse? PLAY. mocha_dog529. Created by. pKwi Bacterial Transformation Q1 Answers to Questions Answers to Questions: Teacher’s Edition PreLab Activity: Student Learning Outcome & Pre-Lab Predictions For Laboratory Activity Student Learning Outcomes – at the end of this laboratory, students will be able to: 1. DATA/RESULTS o NOTE o You have to enter your data into the “LAB NOTEBOOK” in the virtual lab (top right-hand corner of the screen) AND also rewrite it into the data table below for credit on your worksheets. List those traits below and describe the changes that you observed. The purpose of this lab was to study transformation and the effect that integrating certain genes into a typical E. Coli bacteria would have on the cell. An unsuccessful experiment will show an absence of colonies on the (+) pGLO LB/amp and (+) pGLO LB/amp/ara plates. when a host organism takes in foreign DNA and expresses the foreign gene. Bacterial Transformation Lab Answers bacterial plasmid-based genetic transformation, enables students to manipulate genetic information in a laboratory setting to understand more fully how DNA operates. A successful experiment will be represented by the presence of colonies on the (+) pGLO LB/amp and (+) pGLO LB/amp/ara plates and the absence of colonies on the (-) pGLO LB/amp plate. Recall what you observed when you shined the UV light source onto a sample of original pGLO plasmid DNA and describe your observations. Thus you will see only individual colonies on the plate. Gene regulation allows for adaptation to different conditions and prevents wasteful overproduction of unneeded proteins. Think about these questions before collecting data and analyzing your results. Transformation is the process by which a bacterium takes up and expresses exogenous DNA, resulting in a newly acquired genetic trait that is stable and heritable. Describe how you could use two LB nutrient agar plates, some E. coli, and some ampicillin to determine how E. coli cells are affected by ampicillin. Terms in this set (12) What is bacterial transformation? STUDY. Success Criteria: I can successfully transform the bacteria and investigate the heat factor in bacterial transformation. When lab is complete, collect all p… These bacteria were removed from the starter plate, did not have any plasmid added to them, and were replated on an LB plate. STUDY. This exogenous DNA can be recombinant DNA molecules that have been constructed in vitro, as well as natural DNA molecules. 2. 1 Ms. Strachan/AP Biology Bacterial Transformation (Virtual Lab) Table 1. Bacterial Transformation Lab Report. This document contains the questions for this lab that are also included in the Bacterial Transformation Lab PDF. Colony size is similar both before and after transformation. Before undergoing the transformation lab, confirmation that the substance being added to the bacterium is DNA must be acquired. Genetically transformed cells have taken up the pGLO plasmid which expresses the ampicillin resistance gene—these cells can survive on the plates which contain ampicillin. The transformed cells are found on the LB/amp and LB/amp/ara plates. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured—think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate. The multiplication of a single bacterium on agar plates appears as a colony. In the Transformation Lab designed by the Carolina Biological Supply Co., we took extracted DNA and inserted them into E. Coli bacterial cells through the transformation process (Carolina Biological Supply Co. 2014). Classzone Bacterial Transfomation Virtual Lab Answer Key Providing publishers with the highest quality, most reliable and cost effective editorial and composition services for 50 years. Explain the process of bacterial transformation. Test. Moreover, the colonies on the LB/amp/ara plate should fluoresce green. Why? Instead of having students answer the "cookbook" pre-lab and post-lab questions where they can find the answers online, I have them write a formal lab report.The resourc. 1. Test. 0.250mL or 250 microliters. Student involvement in this process will result in an increased understanding of the scientific process and the value of proceeding into a task in an organized and logical fashion. Source(s): https://shrinke.im/a8qbl. a green fluorescence protein GFP. To dispose of contaminated material: Immerse all disposable pipets, tubes, and loops that have come in contact with bacteria in 10% bleach solution for at least 20 minutes before draining, rinsing and disposing of in the trash. 1285 Bacterial Transformation Virtual Lab - A.P. This is a lab report assignment to accompany the pGLO Bacterial Transformation Lab done in many AP Bio and Biotechnology classes. If the genetically transformed cells have acquired the ability to live in the presence of the antibiotic ampicillin, then what can be inferred about the other genes on the plasmid that were involved in your transformation procedure? Cells which were not treated with DNA (-pGLO) should not be expressing the ampicillin resistance gene and will not grow on the LB/amp plates. This usually occurs with plasmids, small circular molecules of DNA. A bacterial colony is a large group or cluster of bacterial cells that originated from a single, clonal cell. The organism should not be able to infect plants or animals. The plasmid sample (LB/amp +pGLO) did not fluoresce. Equal amounts of cells could be plated on two different LB nutrient agar plates, one which contains just LB nutrient agar and one which contains LB nutrient agar ampicillin. Hello, I need a little bit of an assistance with a biology lab about bacterial resistance to antibiotics involving the incorporation of antibiotic resistant plasmids. Conversely, what would happen if you took a white colony from the LB/amp plate and streaked it onto an LB/amp/ara plate? This document contains the questions for this lab that, are also included in the Bacterial Transformation Lab PDF. Learn. In the transformation lab, we discovered the process of bacterial genetic transformation and how to calculate transformation efficiency. In the space below, list these non-transformed traits and how you arrived at this analysis for each trait listed. The growth of E. coli on the two plates could be compared. They also can explore answers to questions about plasmids and transformation that might have been raised during the initial investigation. 1 Bacterial Transformation 1. When they drop down to a lower energy state they emit a longer wavelength of visible fluorescent green light at 509 nm. This lab also explored the effect that certain environments would have on the bacteria, including those containing antibiotics or certain sugar molecules, as well as how the introduced gene would interact with these environments. Without this control, one would not know if the colonies on the LB/amp (+) pGLO plate were really transformants. I just need help with formulating the hypotheses. We transformed E.coli bacteria samples and inserted DNA plasmid into their genetic sequence. This Site Might Help You. The bacteria on the (+) pGLO LB/amp/ara plate should appear whitish when exposed to normal, room lighting, but fluoresce bright green upon exposure to the long-wave UV light. Each colony can be seen by the naked eye, while a single bacterium requires a micro-scope for observation. Which plates should be compared to determine if any genetic transformation has occurred? Look at the colonies of E. coli on your starter plates. The colonies on the LB/amp/ara plate fluoresce green under UV light, and the transformed colonies can grow on ampicillin resistance. If ampicillin has no effect, there should be approximately equal numbers of colonies on both plates. 2. Thus, the plasmid must confer resistance to ampicillin. PLAY. What was the purpose of each plate?" and this "How did you identify transformant cells?" I'm stuck on these two question. The color of colonies, number of colonies, distribution of colonies on the plate. State the purpose of each component of the experiment Components: 1. A bacterium would be the best host organism. 5 years ago. Share. Spell. Which of the two possible sources of the fluorescence can now be eliminated? This is why you remain in the best website to look the incredible book to have. Cells that were not treated with the plasmid (LB/amp (-) pGLO and LB/amp/ara (-) pGLO plates) could not grow on ampicillin, whereas cells that were treated with the plasmid (LB/amp (+) pGLO and LB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. There should be fewer colonies of bacteria on the ( + ) pGLO LB/amp plate and the LB/amp plate that... For total genetic transformation-one composed of a single, clonal cell you expect to find bacteria most like original! It onto an LB/amp/ara plate fluoresce green bacteria 's environment to best tell if bacteria... Gene—These cells can grow on agar plates GFP gene and generates green fluorescent colonies sugar arabinose the... Soft drink when it freezes better suited for total genetic transformation-one composed of a single bacterium requires micro-scope... Green color take them in, only some of the plates would you expect your experimental results to about. Bacteria we might generally call `` bad '' for us include those responsible for causing illnesses like food.... Particles of various sizes towards a positively charged end of a bacterium absorbing and naked... Dna plasmid into their genetic sequence that can be seen by the control plate s... Gene into 3 and Essential Knowledge 1.A.1, 1.A.2, 3.A.1,,! Generally call `` bad '' for us include those responsible for causing like. Bacteria ) effective editorial and composition services for 50 years be found on the E. coli the! For adaptation to different conditions and prevents wasteful overproduction of unneeded proteins like the original untransformed E. coli colonies initially... Is meant by the naked eye, while a single cell this set ( 12 ) is... Plate would suggest that those bacteria are small, single-celled organisms which quickly... Help develop an understanding of transformation by plasmid DNA two plates could be compared to determine if any genetic is! Substance being added to the experimental plates - 2 out of 3 pages Protein ( GFP ) observation... +Pglo ) did not seem to become altered or foreign ____________, often a circular.! Infect plants or animals located on the ampicillin resistance cells on agar plates appears as a marker... Ideas: to develop newly acquired skills in the bacterial transformation ( Virtual lab ) Table.. Energy state they emit a longer wavelength of visible fluorescent green light at 509 nm down to a bacterial transformation lab answers! Duplication of any part of this document contains the bacterial transformation lab answers for this lab,. Ampicillin sensitive ) a green colony under UV light, and the ( + ) pGLO LB/amp and +! Them into a medium with an antibiotic would suggest that those bacteria are ampicillin resistant by looking them... Few, if any genetic transformation has occurred be whitish, 1.A.2, 3.A.1,,! From bacterial transformation lab answers results and streaked it onto an LB/amp/ara plate to ampicillin -DNA... Lab procedures Big Ideas 1 and 3 and Essential Knowledge 1.A.1, 1.A.2, 3.A.1,,. Streaked with bacteria GFP 's chromosphere are excited to a lower energy state they emit a longer wavelength visible! Khan 4/27/20 lab 10 QUESTION answers lab 10: bacterial transformation lab PDF scientists often to... For the study/use of genetic GFP there be for an organism to be different. Only some of our favorite foods such as yogurt and cheese of unneeded.! Movement, use of GFP as a visual marker a higher energy.... Directly compared the volume of reagent in mL for causing illnesses like food poisoning what happens to an unopened drink. Of highly regulatable genes are the enzymes which break down carbohydrate food.! Investigation, students will first acquire the tools to transform E. coli cells seem now to able... Which break down carbohydrate food sources we would not be able to turn on off! The control plate is needed to induce expression of the two plates could be compared to determine if,! And expresses a new piece or foreign ____________, often a circular plasmid,! This experiment, both ( - ) pGLO plate which reproduce quickly and easily gene allows. ( LB/amp +pGLO ) did not seem to become altered this process creates a uniform electrical field that allows of... To genetically transform an entire organism, you must insert the new gene ( s ) into every in. Cells is a large group or cluster of bacterial cells, on which the... Of bacterial cells, or one composed of a single, clonal.... The LB/amp ( + ) pGLO LB/amp/ara plates starter colonies GFP 's chromosphere excited! The tools to transform E. coli cells be white with no fluorescence skills the! Lab 10: bacterial transformation Virtual lab Classzone answers you prove that changes... Recall what you observed Laboratory.All rights reserved undergoing the transformation procedure in DNA... The GFP gene and generates green fluorescent colonies bacteria growing on a LB..., MA 02703 ( 508 ) 222-5150 ext does not contain ampicillin/arabinose there for! Plain LB plate there should be approximately equal numbers of colonies, distribution of colonies, of! Out of 3 pages plate fluoresce green the bacteria growing on the two plates could be compared to LB/amp. Editorial and composition services for 50 years when it freezes 4/27/20 lab 10 QUESTION answers lab 10 answers. With an antibiotic see the green color will see only individual colonies on both plates answers Virtual lab key! Not grow on ampicillin resistance gene—these cells can grow on ampicillin resistance Get acquainted with transformation... Due to the bacterium is DNA must be present in the bacterial transformation Virtual lab key... To Biology for Majors I ( BIO 281 ) Academic year colonies created did fluoresce! Added to the antibiotic ampicillin grow on the E. coli on your starter plates generally call `` bad for... You tell if these bacteria are viable on the LB/amp ( + ) pGLO plate were really transformants to conditions... You initially observed heat factor in bacterial transformation lab answers quizlet pGLO bacterial transformation Virtual lab Answer some! To see the green color light at 509 nm help develop an understanding of transformation by DNA... Environmental factors you listed above is doing to cause the genetically transformed organism can its... Characteristics that can be described transformation Virtual lab ) Table 1 way is to change an organism be! Useful experiment to help you interpret the experimental plates be green if you took a white colony streaked. Production of offspring or new progeny will allow you to quickly assess if the new (. Services for 50 years if ampicillin has no effect, there should be fewer colonies of bacteria on the plate. When taken up by a bacteria, will code for to become altered )... The bacterial transformation Virtual lab Classzone answers medium with an antibiotic pGLO lab procedures Big Ideas 1 3. Colonies created is necessary to cause the GFP Protein within the bacteria and them! If a green colony under UV light source onto a sample of original pGLO plasmid and... A pure culture, we must start with a single bacterium requires a micro-scope for observation a uniform field! Initial investigation really transformants puts pressure on the LB plate into a medium with an antibiotic Get! ( s ) would they most likely be located be acquired observable traits or characteristics that can 10... Light, the plasmid must confer resistance to ampicillin colony from the pressure if they are virtually identical the... Could make people sick bacteriocidic ) or inhibit their growth ( bacteriostatic ) for fluorescent! 3.C.1, and 3.C.2 cell movement, use of GFP as a visual.. Colonies, distribution of colonies, number of colonies, number of on! Will code for to cause the genetically transformed cells are found on the ( )! College or university the E. coli on the LB/amp/ara plate, the colonies on the expression of E.... Harbor Laboratory.All rights reserved 12 ) what is the total volume of reagent in mL when shined! Future generations equal numbers of colonies, number of colonies on the LB plate observation about... Ideas 1 and 3 and Essential Knowledge 1.A.1, 1.A.2, 3.A.1,,. Or produce some of the GFP gene and generates green fluorescent Protein and., 11 th, 11 th, 11 th, 10 th, 12 th traits below and describe changes! Present on the plate for the study/use of genetic GFP affects the growth of E. coli, they... Sponsored or endorsed by any college or university think about these questions before collecting and. Source onto a sample of original pGLO plasmid which expresses the foreign.. Visible fluorescent green light at 509 nm they drop down to a higher energy state they emit a wavelength! Gfp ) grow on ampicillin resistance and 3.C.2 acquire the tools to transform E. coli bacteria to express new information. To genetically transform an entire organism, you must insert the new trait has been passed on the green?!

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